CRAG (Centre for Research in Agricultural Genomics) is developing a high-throughput screening system to assess colonization by the arbuscular mycorrhizal fungi (AMF) Rizhophagus irregularis and the impact of the AMF on resistance to the foliar pathogen Magnaporte oryzae. Expression profiling will be used to identify marker genes for AM-induced disease resistance in rice.
In the University of Torino, colonization and morphology of AMF Rhizophagus irregularis and Funelliformis mosseae are studied in plants grown under the three different conditions: permanent flooding (PF); alternate wetting and drying (AWD); dry condition (DC). AWD plants are in dry period. Plants will be used to identify the genes modulated by different water regime and to analyze the root developmental adaptation.
Morphology of Rhizophagus irregularis arbuscules in rice roots growing in dry (left) and flooding (right) conditions by Vallino et al., 2014
Morphology of Funelliformis mosseae arbuscules in WT (left) and osam18 (right) mutant plants of Oryza sativa (ssp. japonica cv Nipponbare) by Fiorilli et al., 2015
The University of Aberdeen is testing the rice panel for colonization with AMF and for resistance to root knot nematodes. Candidate genes for these biotic interactions will be identified.