GreenRice

Research on mycorrhiza and resistance to nematodes and blast infestation

CRAG (Centre for Research in Agricultural Genomics) is developing a high-throughput screening system to assess colonization by the arbuscular mycorrhizal fungi (AMF) Rizhophagus irregularis and the impact of the AMF on resistance to the foliar pathogen Magnaporte oryzae. Expression profiling will be used to identify marker genes for AM-induced disease resistance in rice.

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Colonization of rice roots by the AM fungus Rhizophagus irregularis (microscopical observations of trypan blue-stained roots). Photos by Dr. Sonia Campo (CRAG)

Colonization of rice roots by the AM fungus Rhizophagus irregularis (microscopical observations of trypan blue-stained roots). Photos by Dr. Sonia Campo (CRAG)

 

 

 

 

 

 

 

 

 

 

In the University of Torino, colonization and morphology of AMF Rhizophagus irregularis and Funelliformis mosseae are studied in plants  grown under the three different conditions: permanent flooding (PF); alternate wetting and drying (AWD); dry condition (DC). AWD plants are in dry period. Plants will be used to identify the genes modulated by different water regime and to analyze the root developmental adaptation.

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Morphology of Rhizophagus irregularis arbuscules in rice roots growing in dry (left) and flooding (right) conditions by Vallino et al., 2014

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Morphology of Funelliformis mosseae arbuscules in WT (left) and osam18 (right) mutant plants of Oryza sativa (ssp. japonica cv Nipponbare) by Fiorilli et al., 2015

 

The University of Aberdeen is testing the rice panel for colonization with AMF and for resistance to root knot nematodes. Candidate genes for these biotic interactions will be identified.

Dr Roshi Shrestha (University of Aberdeen) harvesting F3 seeds of a cross made to map nematode resistance for WP3

Dr Roshi Shrestha (University of Aberdeen) harvesting F3 seeds of a cross made to map nematode resistance

 

 

 

 

 

GreenRice:
Sustainable and environmental friendly rice cultivation systems in Europe is funded by the FACCE-ERA-NET+ on Climate Smart Agriculture.

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